| Author | Babar, Sheikh Md. Enayetul |
| Call Number | AIT Thesis no. BP-01-02 |
| Subject(s) | Bifidobacterium
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of
Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-01-02 |
| Abstract | Bifidobacterium lactis was cultured in different media at different fermentation conditions to
investigate its growth on different substrates. Different types of carbohydrate sources such as
glucose, fructooligosaccharides (FOS), soymilk, and soy broth were investigated. Various
composition and fermentation methods were tried in this work. It has been observed that
vaiiation of carbohydrate source in the media had considerable effects on cell growth. Media
containing 2% glucose + 2% soymilk (10 times diluted) and 1 % glucose + 1 % FOS as carbon
source were found to best media for these cells growth. Inocula concentration and pre-cultures
had considerable effects on cell growth. Among the different conditions, pre-cultures and seed
cultures having same media with that of final bioreactor also showed better result in term of
cell growth. The cells were tested to expose a media containing 100 µg/ml of ampicillin and
found to have some resistance to this antibiotic. A complex carbohydrate like chitosan
oligosaccharide was found to be a good growth media for this species, where growth was
higher than that of the glucose media. The reactor volume was found to be a factor that
effected the cell growth. Cells were encapsulated in calcium alginate, chitosan and calcium
alginate/chitosan mixers to check their survivability in simulated gastric juice. It was found
that encapsulation by calcium alginate yielded better survivability than its free form.
Determination of survivability in the other two matrices was difficult as they did not swell
easily and release the encapsulated microorganisms. It was also found that cells produce
acetic acid and lactic acid on their growth. The net lactic acid production was higher than that
of acetic acid production, which indicated that in its metabolic pathway the main end product
is lactic acid. |
| Year | 2001 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-01-02 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip K.; |
| Examination Committee(s) | Jindal, V.K.;Athapol Noomhorm ; |
| Scholarship Donor(s) | Deutscher Akademischer Austauschdienst
(DAAD) ; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2001 |