| Author | Thi Thi Nge |
| Call Number | AIT Thesis no. BP-98-7 |
| Subject(s) | Chitin
|
| Note | A thesis submitted in partial fulfillment of the requirements for
the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | The source of chitin deacetylase used in this study was Absidia coerulea (IFO 5301).
The parameters studied in this work were the cultivation conditions for increasing enzyme
production. Size and age of inoculum, time of incubation, composition of culture medium and
pH effect on production of chitin deacetylase were investigated in shake flask fermentations.
In the present study the sample which had 58.8% DD chitosan (1.5%) gave the highest activity
as compared to glucose and N-acetyl D-glucosamine. However, the method of preparation of
the chitosan sample is an important criteria for enzyme activity. Cultivation in glucose 2%
medium and N-acetyl D-glucosamine 2% medium with initial pH 4.5 was optimal. The effect
of peptone on enzyme production medium was also studied. The optimum inoculum age and
inoculum size were found to be 5-day and 1.32 x 107 spores/ml respectively. The total activity
of chitin deacetylase in culture filtrate of Absidia coerulea was maximal after 24 h in glucose
2% medium, after 32 h in 2% N-acetyl D-glucosamine medium, and 72 h in 1.5% chitosan
58.8% DD medium respectively. The total activity obtained from reactor study was nearly
same value as shake flask fermentation due to wall growth and inadequate mixing condition.
The morphology of the organism, which affects fermentation, is dependent on a number of
parameters like nutrient source, pH, temperature, etc. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip K.; |
| Examination Committee(s) | Stevens, Willem F. ;Suwalee Chandrkrachang ;Pakorn Nuchnoi ;Montet, Didier; |
| Scholarship Donor(s) | Asian Development Bank-Japan Scholarship Program; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |