| Author | Anal, Anil Kumar |
| Call Number | AIT Thesis no. BP-98-12 |
| Subject(s) | Chitosan
|
| Note | A thesis submitted in partial fulfillment of the requirements
for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | In this study, both chitosan beads and chitosan membrane have been investigated for
applications in systems of controlled release of phaimaceuticals. Chitosan beads can be prepai-ed
by microencapsulation of chitosan droplets. Microencapsulation comprises various technologies
that can be used to coat small particles of solid or droplets of liquid dispersion with a thin
coating layer. The dropping of dissolved chitosan into a solution of tripolyphosphate (TPP) leads
instantaneously to the formation of beads by ionotropic gelation without using any sophisticated
instruments. Conditions of microencapsulation have been investigated to optimize bead
formation and entrapment of the model protein, bovine serum albumin (BSA). The speed of
droppings, a slight stirring speed of TPP solution, 5-7 cm dropping distance from the surface of
TPP solution and the type, concentration, and viscosity of chitosan solution were fotmd the
major influensive parameters to form smooth and spherical chitosan beads. Crosslinking
between chitosan and TPP occurs within 15 minutes. The pH 7.2 ofTPP was found the optimum
pH for the maximum entrapment of bovine serum albumin (BSA), which was used as model
drug for this study. The degree of deacetylation of chitosan showed an influensive on the
entrapment of BSA while the concentration ofTPP, viscosity of chitosan solution ai1d more than
3% load of BSA did not have any effect. The chitosan beads/microcapsules were found to swell
in acidic medium (0.1 N HCl). The release of BSA from the chitosan microcapsules was found
depending upon the pH of the dissolution medium, drying condition, and the type of chitosan.
Chitosan membrane prepared from chitosan with a 95% degree of deacetylation showed
similar behavior as of commercially available cellulose membrane. The equilibrium dialysis for
all model drugs aspirin, phenobarbitone Na salt, and Sulfanilamide occurred within two hours in
both cellulose and chitosan membranes. The fraction of unbound drugs could be determined by
using chitosan membrane. The unbound fractions of drugs obtained by using commercial
regenerated cellulose membrane were comparable with those obtained by using chitosan
membrane. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Stevens, Willem F. ; |
| Examination Committee(s) | Korbtham Sathirakul ;Suwalee Chandrkrachang ;Rakshit, Sudip Kumar, ;Montet, Didier ;Pakorn Nuchnoi ; |
| Scholarship Donor(s) | Government of Austria; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |