| Author | Maskey, Sabin Raj |
| Call Number | AIT Thesis no. BP-98-14 |
| Subject(s) | Transglutaminases
|
| Note | A thesis submitted in partial fulfillment of the requirements
for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | Initial shake flask fermentation studies on the production of transglutaminase enzyme using
Streptoverticillium ladakanum indicated that product formation occurred with a short lag as
compared to biomass development. An optimal initial glycerol concentration and initial pH was
also optimised for a batch culture in shake flasks using Response Surface Methodology and it
was found that glycerol concentration of 1.8% and pH of 7.126 gave the high values. A
complete program was developed using Macsyma for Pontryagin's Continuous Maximum
Principle which can be used for optimal control problems. Temperature was found to be a
critical parameter affecting enzyme production. An optimal temperature profile was determined
using Pontryagin's Continuous Maximum Principle. Semi-empirical process models were used
to minimize complications in the computation. |
| Year | 1998 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Stevens, Willem F. ;Pakorn Nuchnoi ;Suwalee Chandrakrachang ;Montet, Didier; |
| Scholarship Donor(s) | His Majesty King Bhumibol Adulyadej of
Thailand .; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1998 |