| Author | Peng, Fu-chuo |
| Call Number | AIT Thesis no. EV-84-8 |
| Subject(s) | Mycotoxins--Physiological effect
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Abstract | The procedures of isolation and purification of B' were
found to be similar to those of TRD. B' was highly soluble in
chloroform and less soluble in n-hexane. These satisfies the use
of chloroform as an extractant and a-hexane as a precipitant. The chloroform:acetone solvent system for clean-up step was also the same as for TRB. As the benzene-ehtylacetate was shown to be the
best system for separating TRA and TRB, the same solvent system,
benzene-ehtylacetate (50 :5 V/V) could be effectively used to
separate TRB and B'.
B' has a maximal UV absorption at 219 nm and 331 nm which
are similar to those of TRB but B' of E219/E331 is 1.68 which is
lower than that of TBB. The eleven protons of aryl moiety of TRB including three methoxyl Ar-OCH3 and two Ar-H2 were also found in PMR of B'. One proton of C(B) of TRB was equivalent of one
proton of B'. IR at 1967 cm-1 in B' as well as in TRB is typical
for conjugated Ketone carbonyl group. IR at 1127cm-1 in B'
demonstrated the presence of c-o-c stretching. A series of
fragment ions of TRB such as m/e 195, 2·10, 237, 278, 291 and 345
were also found in B'. 'file results were interpreted that B'· has
similar structure of A, B, and C ring as TRB. From high
resolution mass spectrum analysis shown that the molecular composition of B' was c,. H34 O10 while that of TRB was C 29 H34 O9.
Thus the difference of the molecular composition of these two compounds was 16 (0). However, the exact structure of B'· should be
studied with the X-ray crystallography.
Although 1 mg up to 2 mg of B' did not induce tremors or
other symptoms in mice by 1.p. injection. B' could augment twitch
tension of mice phrenic nerve-·diaphragm preparation by indirect
stimulation at the concentration of 3 pg/mL. The activity is
approximately 1/750 of TRB. No cytotoxicity was observed in
sister chromatid exchange __ in Chi11ese hamster ovaries cells
culture in vivo at the concentration of 100 mM, and no mutagenicity was found from the sister chromatid exchange test. and Ames' test. |
| Year | 1984 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Chongrak Polprasert
|
| Examination Committee(s) | I, Fude ;Samorn Muttamara |
| Scholarship Donor(s) | Carl Duisberg Gesellschaft |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 1984 |