Modulatory role of MYO-inositol on growth, osmoregulaton, and stress tolerance of macrobrachium rosenbergii across salinity gradients | |
| Author | Bagde, Sakshi Mukund |
| Call Number | AIT Thesis no.AQ-25-04 |
| Subject(s) | Macrobrachium rosenbergii Osmoregulation Aquatic ecology |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management |
| Publisher | Asian Institute of Technology |
| Abstract | This study evaluates the effects of dietary myo-inositol (MI) supplementation on growth, survival, osmoregulation, antioxidant defense, and stress tolerance in giant freshwater prawn (Macrobrachium rosenbergii) post-larvae exposed to varying salinity levels (0–12 ppt) Salinity stress limits M. rosenbergii culture by increasing osmoregulatory costs and oxidative damage, making dietary interventions like myo-inositol essential for enhancing antioxidant defense and physiological resilience. Two 30-day feeding trials were conducted. In Trial 1, three MI inclusion levels (0.35%, 0.45%, and 0.55%) were tested under 0 and 10 ppt salinity conditions to determine the optimal dose. Post-larvae were fed a commercial diet containing 35% crude protein, and all MI-supplemented groups showed significantly higher weight gain, improved feed conversion ratio (FCR), and better survival than controls (p < 0.001 significant differences observed among treatments). The 0.55% MI group at 0 ppt exhibited the greatest weight gain (3.583 g vs. 1.113 g in control) and superior FCR (1.39 vs. 1.73). Regression analysis revealed a linear dose-response, identifying 0.55% MI as the most effective inclusion level within the tested range. In Trial 2, the 0.55% MI diet was evaluated at broader salinities (0, 6, and 12 ppt). 0.55% MI significantly enhanced growth across all salinities (p < 0.01). At 6 ppt, average weight gain nearly doubled (5.05 g vs. 2.60 g), biomass increased from 44.60 g to 79.30 g, and FCR improved from 2.23 to 1.23. Survival declined with increasing salinity but remained slightly higher in supplemented groups (p > 0.05). Physiological benefits included a ~50% reduction in ATPase activity at 6 ppt (p < 0.001), a two- to three-fold increase in antioxidant capacity at 0 and 6 ppt (p < 0.001), and markedly lower glucose concentrations (0.186 vs. 0.287 mg/ml at 6 ppt, p < 0.001), reflecting better metabolic regulation under salinity stress. Overall, MI supplementation enhanced physiological resilience across environmental salinity gradients. Supplementation at 0.55% improved growth, feed efficiency, and stress tolerance, positioning MI as a promising nutritional strategy to strengthen osmoregulatory and antioxidant defenses in M. rosenbergii. These findings support MI’s potential role in improving aquaculture productivity and sustainability, particularly under climate-driven salinity fluctuations. |
| Year | 2025 |
| Type | Thesis |
| School | School of Environment, Resources, and Development |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Salin, Krishna R.; |
| Examination Committee(s) | Ha, Thanh Dong;Tsusaka, Takuji W.; |
| Scholarship Donor(s) | AIT Partial Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2025 |